GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM4483896 Query DataSets for GSM4483896
Status Public on May 31, 2020
Title LNM8
Sample type RNA
Source name LNM8
Organism Homo sapiens
Characteristics tissue: Lymph node metastasis
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
Hybridization protocol Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
Description microRNA expression of lymph node metastatic breast cancer.
Data processing The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
Submission date Apr 17, 2020
Last update date Jun 01, 2020
Contact name Nehad M Alajez
Organization name King Saud University College of Medicine
Department Anatomy
Lab Stem Cell Unit
Street address 1st floor College of Medicne
City Riyadh
ZIP/Postal code 11461
Country Saudi Arabia
Platform ID GPL21576
Series (2)
GSE148846 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients
GSE148848 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00015259 2.250580405
A_25_P00010378 1.650037225
A_25_P00015257 6.13322536
A_25_P00015256 1.755636819
A_25_P00015255 1.702409645
A_25_P00015254 10.9793507
A_25_P00010373 7.681293801
A_25_P00010372 2.12840133
A_25_P00010371 3.295883243
A_25_P00010370 6.137128834
A_25_P00015781 8.0244909
A_25_P00015780 6.457991444
A_25_P00015783 4.140129455
A_25_P00015782 3.799054777
A_25_P00015785 2.459871749
A_25_P00015784 6.825031966
A_25_P00015787 3.757720597
A_25_P00015786 6.582853102
A_25_P00015789 3.066088855
A_25_P00015788 6.977522602

Total number of rows: 5937

Table truncated, full table size 155 Kbytes.

Supplementary file Size Download File type/resource
GSM4483896_LNM8.txt.gz 2.8 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap