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Sample GSM4483890 Query DataSets for GSM4483890
Status Public on May 31, 2020
Title LNM12
Sample type RNA
Source name LNM12
Organism Homo sapiens
Characteristics tissue: Lymph node metastasis
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
Hybridization protocol Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
Description microRNA expression of lymph node metastatic breast cancer.
Data processing The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
Submission date Apr 17, 2020
Last update date Jun 01, 2020
Contact name Nehad M Alajez
Organization name King Saud University College of Medicine
Department Anatomy
Lab Stem Cell Unit
Street address 1st floor College of Medicne
City Riyadh
ZIP/Postal code 11461
Country Saudi Arabia
Platform ID GPL21576
Series (2)
GSE148846 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients
GSE148848 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00015259 4.837786162
A_25_P00010378 4.425100481
A_25_P00015257 4.263770242
A_25_P00015256 3.770990943
A_25_P00015255 1.443324673
A_25_P00015254 4.337689302
A_25_P00010373 1.898073918
A_25_P00010372 4.396042654
A_25_P00010371 4.322020793
A_25_P00010370 3.460912939
A_25_P00015781 4.026079984
A_25_P00015780 0.310670182
A_25_P00015783 0.961078873
A_25_P00015782 3.337764631
A_25_P00015785 3.357826474
A_25_P00015784 1.395754015
A_25_P00015787 1.773084508
A_25_P00015786 1.388719885
A_25_P00015789 3.301840726
A_25_P00015788 7.894950129

Total number of rows: 5937

Table truncated, full table size 155 Kbytes.

Supplementary file Size Download File type/resource
GSM4483890_LNM12.txt.gz 2.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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