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Sample GSM4483889 Query DataSets for GSM4483889
Status Public on May 31, 2020
Title BC13
Sample type RNA
Source name BC13
Organism Homo sapiens
Characteristics tissue: Paired primary breast cancer
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
Hybridization protocol Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
Description microRNA expression of primary breast cancer.
Data processing The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
Submission date Apr 17, 2020
Last update date Jun 01, 2020
Contact name Nehad M Alajez
Organization name King Saud University College of Medicine
Department Anatomy
Lab Stem Cell Unit
Street address 1st floor College of Medicne
City Riyadh
ZIP/Postal code 11461
Country Saudi Arabia
Platform ID GPL21576
Series (2)
GSE148846 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients
GSE148848 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00015259 5.050410601
A_25_P00010378 3.944194985
A_25_P00015257 5.91338208
A_25_P00015256 6.744875347
A_25_P00015255 1.803052629
A_25_P00015254 4.049180736
A_25_P00010373 2.533974501
A_25_P00010372 3.501831784
A_25_P00010371 4.764589564
A_25_P00010370 3.692344703
A_25_P00015781 3.480299101
A_25_P00015780 1.271911751
A_25_P00015783 2.518107903
A_25_P00015782 1.715311891
A_25_P00015785 1.946808624
A_25_P00015784 2.12840133
A_25_P00015787 2.158885407
A_25_P00015786 3.417398364
A_25_P00015789 3.880586495
A_25_P00015788 -1.109572483

Total number of rows: 5937

Table truncated, full table size 155 Kbytes.

Supplementary file Size Download File type/resource
GSM4483889_BC13.txt.gz 2.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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