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Sample GSM4483888 Query DataSets for GSM4483888
Status Public on May 31, 2020
Title LNM13
Sample type RNA
Source name LNM13
Organism Homo sapiens
Characteristics tissue: Lymph node metastasis
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
Hybridization protocol Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
Description microRNA expression of lymph node metastatic breast cancer.
Data processing The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
Submission date Apr 17, 2020
Last update date Jun 01, 2020
Contact name Nehad M Alajez
Organization name King Saud University College of Medicine
Department Anatomy
Lab Stem Cell Unit
Street address 1st floor College of Medicne
City Riyadh
ZIP/Postal code 11461
Country Saudi Arabia
Platform ID GPL21576
Series (2)
GSE148846 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients
GSE148848 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00015259 4.35933233
A_25_P00010378 6.220428532
A_25_P00015257 4.86077433
A_25_P00015256 3.775532738
A_25_P00015255 0.493640366
A_25_P00015254 4.072810491
A_25_P00010373 4.773675119
A_25_P00010372 3.301284723
A_25_P00010371 4.438200947
A_25_P00010370 2.343173816
A_25_P00015781 3.555685783
A_25_P00015780 0.086945955
A_25_P00015783 4.064715052
A_25_P00015782 3.88656519
A_25_P00015785 2.606073932
A_25_P00015784 0.796140498
A_25_P00015787 3.496548186
A_25_P00015786 3.789927171
A_25_P00015789 3.862956115
A_25_P00015788 7.657438974

Total number of rows: 5937

Table truncated, full table size 155 Kbytes.

Supplementary file Size Download File type/resource
GSM4483888_LNM13.txt.gz 2.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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