|
| Status |
Public on Oct 19, 2020 |
| Title |
whole cells, ITC_APR |
| Sample type |
SRA |
| |
|
| Source name |
whole cell
|
| Organism |
Candidozyma auris |
| Characteristics |
cell type: whole cell
treatment: Itraconazole/Aprepitant combination (1/10 µg/ml)
|
| Treatment protocol |
Exponential-phase cultures of C. auris AR0390 were treated with either DMSO (1%), aprepitant (10 µg/ml), itraconazole (1 µg/ml), or a combination of both drugs.
|
| Growth protocol |
Candida auris (AR0390) cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion Ribopure yeast kit
Illumina TruSeq kit
2x150 on a NovaSeq 6000
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
fastp was used to observe read quality
fastp was used to trim data
HISAT2 (v2.1) to align reads to the reference genome
FeatureCounts (v2.1) was used to count reads mapping to genes
edgeR Bioconductor package (v3.16.5) to identify differentially expressed genes
Genome_build: Cand_auris_B11221_V1
Supplementary_files_format_and_content: APR.RNAseq.genecounts.txt
|
| |
|
| Submission date |
Apr 15, 2020 |
| Last update date |
Oct 19, 2020 |
| Contact name |
Nadia Marie Atallah |
| Organization name |
Purdue University
|
| Department |
Center for Cancer Research
|
| Street address |
201 S. University St
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
| |
|
| Platform ID |
GPL28368 |
| Series (1) |
| GSE148749 |
Aprepitant, an antiemetic agent, displays potent synergistic interactions with azole drugs against emerging multidrug-resistant Candida auris |
|
| Relations |
| BioSample |
SAMN14602837 |
| SRA |
SRX8120239 |
