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Sample GSM4474982 Query DataSets for GSM4474982
Status Public on Sep 26, 2022
Title SLE51-post Bcell
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: Bcell
date measured: 14.01.31
age: 21
Sex: F
sex (1 vs 2): 2
sledai-2k: 4
antidna(ria)antibody titer(u/ml:<6): 7
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 0
pre / post: post
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.28795767
ETG10_236652 0.008047104
ETG10_234183 0.16719675
ETG10_195139 -0.1955638
ETG10_13482 -0.06045246
ETG09_48764 -0.27056074
ETG09_35454 -0.20434284
ETG09_205211 -0.17299938
ETG08_142674 0.002833843
ETG07_105829 0.14881086
ETG05_66023 0.44614887
ETG05_36762 0.16918182
ETG04_27747 -0.18475914
ETG02_36680 -0.15133953
ERCC-00171_229 -0.029054165
ERCC-00170_100 0.7726326
ERCC-00168_248 -0.22798824
ERCC-00165_60 -0.1720829
ERCC-00164_60 -0.19553757
ERCC-00163_81 -0.26748085

Total number of rows: 50739

Table truncated, full table size 1224 Kbytes.

Supplementary file Size Download File type/resource
GSM4474982_US91700289_253949414873_S01_GE1_107_Sep09_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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