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Sample GSM4474974 Query DataSets for GSM4474974
Status Public on Sep 26, 2022
Title SLE24 Bcell
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: Bcell
date measured: 14.01.22
age: 23
Sex: F
sex (1 vs 2): 2
sledai-2k: 6
antidna(ria)antibody titer(u/ml:<6): 15
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 0
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.3844576
ETG10_236652 0.34533882
ETG10_234183 0.16467476
ETG10_195139 0.38716507
ETG10_13482 0.2991867
ETG09_48764 0.3808074
ETG09_35454 0.39321423
ETG09_205211 0.39573002
ETG08_142674 0.26644325
ETG07_105829 0.16266632
ETG05_66023 0.12253237
ETG05_36762 -0.031929493
ETG04_27747 0.3090315
ETG02_36680 0.37715912
ERCC-00171_229 0.40292692
ERCC-00170_100 0.32322264
ERCC-00168_248 0.3586445
ERCC-00165_60 0.39796352
ERCC-00164_60 0.41780853
ERCC-00163_81 0.32098675

Total number of rows: 50739

Table truncated, full table size 1216 Kbytes.




Supplementary file Size Download File type/resource
GSM4474974_US91700289_253949414872_S01_GE1_107_Sep09_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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