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Sample GSM4474973 Query DataSets for GSM4474973
Status Public on Sep 26, 2022
Title SLE27 Bcell
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: Bcell
date measured: 14.01.22
age: 30
Sex: M
sex (1 vs 2): 1
sledai-2k: 0
antidna(ria)antibody titer(u/ml:<6): 2
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 0
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.015317917
ETG10_236652 0.048180103
ETG10_234183 0.101361275
ETG10_195139 0.14365244
ETG10_13482 -0.053843975
ETG09_48764 0.0667882
ETG09_35454 0.085544586
ETG09_205211 0.15881538
ETG08_142674 -0.06485462
ETG07_105829 -0.004354477
ETG05_66023 -0.11225796
ETG05_36762 0.24546623
ETG04_27747 0.13213253
ETG02_36680 0.09648991
ERCC-00171_229 0.0634613
ERCC-00170_100 0.84515333
ERCC-00168_248 0.12295532
ERCC-00165_60 0.103014946
ERCC-00164_60 0.09510994
ERCC-00163_81 0.044851303

Total number of rows: 50739

Table truncated, full table size 1227 Kbytes.

Supplementary file Size Download File type/resource
GSM4474973_US91700289_253949414872_S01_GE1_107_Sep09_2_2.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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