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Sample GSM4474972 Query DataSets for GSM4474972
Status Public on Sep 26, 2022
Title SLE1 Bcell
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: Bcell
date measured: 14.01.22
age: 31
Sex: M
sex (1 vs 2): 1
sledai-2k: 4
antidna(ria)antibody titer(u/ml:<6): 10
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 0
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.40873718
ETG10_236652 0.5656605
ETG10_234183 0.62573814
ETG10_195139 0.6439748
ETG10_13482 0.45256805
ETG09_48764 0.5782814
ETG09_35454 0.59696484
ETG09_205211 0.6028614
ETG08_142674 0.48058033
ETG07_105829 0.45869637
ETG05_66023 0.29332733
ETG05_36762 0.6677704
ETG04_27747 0.5025344
ETG02_36680 0.6007786
ERCC-00171_229 0.5916891
ERCC-00170_100 -0.06859684
ERCC-00168_248 0.593812
ERCC-00165_60 0.6128001
ERCC-00164_60 0.60195255
ERCC-00163_81 0.57794666

Total number of rows: 50739

Table truncated, full table size 1205 Kbytes.

Supplementary file Size Download File type/resource
GSM4474972_US91700289_253949414872_S01_GE1_107_Sep09_2_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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