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Sample GSM4474968 Query DataSets for GSM4474968
Status Public on Sep 26, 2022
Title HC27 Bcell
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: Bcell
date measured: 14.01.22
age: 36
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.5392871
ETG10_236652 -0.27847767
ETG10_234183 -0.17418861
ETG10_195139 -0.21035767
ETG10_13482 0.13546944
ETG09_48764 -0.15401316
ETG09_35454 -0.26229715
ETG09_205211 -0.26514244
ETG08_142674 -0.07609606
ETG07_105829 -0.32860327
ETG05_66023 -0.5335617
ETG05_36762 0.2858448
ETG04_27747 -0.16323328
ETG02_36680 -0.24187183
ERCC-00171_229 -0.2595024
ERCC-00170_100 1.233511
ERCC-00168_248 0.04997778
ERCC-00165_60 -0.26959658
ERCC-00164_60 -0.2268014
ERCC-00163_81 -0.24533606

Total number of rows: 50739

Table truncated, full table size 1222 Kbytes.

Supplementary file Size Download File type/resource
GSM4474968_US91700289_253949414872_S01_GE1_107_Sep09_1_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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