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Sample GSM4474966 Query DataSets for GSM4474966
Status Public on Sep 26, 2022
Title SLE18 PBMC
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: PBMC
date measured: 14.01.16
age: 30
Sex: F
sex (1 vs 2): 2
sledai-2k: 8
antidna(ria)antibody titer(u/ml:<6): 24
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 0
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.1540513
ETG10_236652 -0.077858925
ETG10_234183 -0.28477955
ETG10_195139 -0.07455349
ETG10_13482 -0.21284771
ETG09_48764 -0.12620449
ETG09_35454 -0.08111
ETG09_205211 -0.054283142
ETG08_142674 -0.25673962
ETG07_105829 -0.28660917
ETG05_66023 -0.2800355
ETG05_36762 -0.39154768
ETG04_27747 -0.16676044
ETG02_36680 -0.076880455
ERCC-00171_229 -0.14017296
ERCC-00170_100 -0.4193468
ERCC-00168_248 -0.13847876
ERCC-00165_60 -0.07864094
ERCC-00164_60 -0.08613205
ERCC-00163_81 -0.14956284

Total number of rows: 50739

Table truncated, full table size 1229 Kbytes.




Supplementary file Size Download File type/resource
GSM4474966_US91700289_253949414864_S01_GE1_107_Sep09_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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