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Sample GSM4474965 Query DataSets for GSM4474965
Status Public on Sep 26, 2022
Title SLE43-post PBMC
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: PBMC
date measured: 14.01.16
age: 66
Sex: F
sex (1 vs 2): 2
sledai-2k: 2
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 1
pre / post: post
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.51599264
ETG10_236652 -0.11716318
ETG10_234183 0.039941788
ETG10_195139 -0.089408875
ETG10_13482 -0.2662258
ETG09_48764 -0.10949516
ETG09_35454 -0.07831383
ETG09_205211 -0.08962965
ETG08_142674 -0.016410828
ETG07_105829 -0.049759865
ETG05_66023 -0.1454258
ETG05_36762 -0.2123766
ETG04_27747 -0.14588785
ETG02_36680 -0.09406662
ERCC-00171_229 -0.14795971
ERCC-00170_100 -0.231596
ERCC-00168_248 -0.08738136
ERCC-00165_60 -0.091202736
ERCC-00164_60 -0.106806755
ERCC-00163_81 -0.16408348

Total number of rows: 50739

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM4474965_US91700289_253949414864_S01_GE1_107_Sep09_2_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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