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Sample GSM4474962 Query DataSets for GSM4474962
Status Public on Sep 26, 2022
Title HC32 PBMC
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: PBMC
date measured: 14.01.16
age: 39
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 1.1650786
ETG10_236652 -0.046900272
ETG10_234183 0.2402358
ETG10_195139 -0.05653143
ETG10_13482 0.17600727
ETG09_48764 -0.07737303
ETG09_35454 -0.054374695
ETG09_205211 -0.053165913
ETG08_142674 -0.13925648
ETG07_105829 0.6127691
ETG05_66023 0.003005981
ETG05_36762 0.06467056
ETG04_27747 -0.097753525
ETG02_36680 -0.021695614
ERCC-00171_229 -0.10367966
ERCC-00170_100 0.14486885
ERCC-00168_248 -0.12119007
ERCC-00165_60 -0.059798717
ERCC-00164_60 -0.06609964
ERCC-00163_81 -0.06923294

Total number of rows: 50739

Table truncated, full table size 1228 Kbytes.




Supplementary file Size Download File type/resource
GSM4474962_US91700289_253949414864_S01_GE1_107_Sep09_1_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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