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Sample GSM4474949 Query DataSets for GSM4474949
Status Public on Sep 26, 2022
Title SLE16 memory B
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: memory B
date measured: 13.12.26
age: 59
Sex: F
sex (1 vs 2): 2
sledai-2k: 2
antidna(ria)antibody titer(u/ml:<6): 8
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 1
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 1.799293
ETG10_236652 0.25171137
ETG10_234183 0.25678396
ETG10_195139 0.25915432
ETG10_13482 0.104204655
ETG09_48764 0.30785036
ETG09_35454 0.27056646
ETG09_205211 0.27373314
ETG08_142674 0.10981274
ETG07_105829 0.15635872
ETG05_66023 0.43666458
ETG05_36762 0.028591156
ETG04_27747 0.18189049
ETG02_36680 0.26777935
ERCC-00171_229 0.26363754
ERCC-00170_100 2.94879
ERCC-00168_248 0.21049881
ERCC-00165_60 0.27592802
ERCC-00164_60 0.28866005
ERCC-00163_81 0.19016361

Total number of rows: 50739

Table truncated, full table size 1213 Kbytes.

Supplementary file Size Download File type/resource
GSM4474949_US91700289_253949414862_S01_GE1_107_Sep09_2_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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