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Sample GSM4474939 Query DataSets for GSM4474939
Status Public on Sep 26, 2022
Title HC32 memory B
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: memory B
date measured: 13.12.19
age: 39
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.31712103
ETG10_236652 -0.20211029
ETG10_234183 -0.34452152
ETG10_195139 -0.17158318
ETG10_13482 -0.034876823
ETG09_48764 -0.11589861
ETG09_35454 -0.1627407
ETG09_205211 -0.1585722
ETG08_142674 -0.20825672
ETG07_105829 0.019751549
ETG05_66023 -0.23260784
ETG05_36762 0.2742777
ETG04_27747 -0.12440729
ETG02_36680 -0.11471462
ERCC-00171_229 -0.04584551
ERCC-00170_100 0.4703803
ERCC-00168_248 -0.20679426
ERCC-00165_60 -0.16701508
ERCC-00164_60 -0.17762184
ERCC-00163_81 -0.24418736

Total number of rows: 50739

Table truncated, full table size 1225 Kbytes.

Supplementary file Size Download File type/resource
GSM4474939_US91700289_253949412006_S01_GE1_107_Sep09_1_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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