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Sample GSM4474914 Query DataSets for GSM4474914
Status Public on Sep 26, 2022
Title HC31 naive B
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: naive B
date measured: 13.11.29
age: 36
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -1.8110509
ETG10_236652 -0.4099555
ETG10_234183 -0.5066209
ETG10_195139 -0.47772503
ETG10_13482 -0.43971825
ETG09_48764 -0.42006016
ETG09_35454 -0.45506573
ETG09_205211 -0.4448099
ETG08_142674 -0.37370968
ETG07_105829 -0.6256137
ETG05_66023 -0.7892041
ETG05_36762 -0.55290985
ETG04_27747 -0.48941612
ETG02_36680 -0.4619422
ERCC-00171_229 -0.38646746
ERCC-00170_100 0.34090137
ERCC-00168_248 -0.34734154
ERCC-00165_60 -0.45149565
ERCC-00164_60 -0.4700179
ERCC-00163_81 -0.3746462

Total number of rows: 50739

Table truncated, full table size 1222 Kbytes.

Supplementary file Size Download File type/resource
GSM4474914_US91700289_253949412045_S01_GE1_107_Sep09_1_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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