|
Status |
Public on Jun 30, 2011 |
Title |
5720 B10.D2->balb rag2-/- IL4Ra-/-(new) |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
B10.D2->balb rag2-/- IL4Ra-/-
|
Organism |
Mus musculus |
Characteristics |
tissue: skin
|
Treatment protocol |
Skin harvested from cGVHD mice at 2 weeks post splenocyte transfer.
|
Growth protocol |
cGVHD mice generated by splenocyte transfer of B10.D2 into balb/c; syngeneic controls balb/c into balb/c.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA isolated using Trizol methods; cleaned up with Qiagen RNeasy mini-columns.
|
Label |
Cy3
|
Label protocol |
Agilent Linear Amplification Low RNA Input Kit.
|
|
|
Channel 2 |
Source name |
Universal Mouse Reference
|
Organism |
Mus musculus |
Characteristics |
cell line: multiple
|
Treatment protocol |
Skin harvested from cGVHD mice at 2 weeks post splenocyte transfer.
|
Growth protocol |
cGVHD mice generated by splenocyte transfer of B10.D2 into balb/c; syngeneic controls balb/c into balb/c.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA isolated using Trizol methods; cleaned up with Qiagen RNeasy mini-columns.
|
Label |
Cy5
|
Label protocol |
Agilent Linear Amplification Low RNA Input Kit.
|
|
|
|
Hybridization protocol |
Agilent Protocols and Hybridization Kits.
|
Scan protocol |
GenePix 400B scanner (Axon Instruments).
|
Description |
Universal Mouse Reference RNA (Stratagene).
|
Data processing |
Feature extraction with GenePix 5.1 software.
|
|
|
Submission date |
Sep 01, 2009 |
Last update date |
Jun 30, 2011 |
Contact name |
Jennifer L Sargent |
E-mail(s) |
jennifer.sargent@gmail.com
|
Organization name |
Dartmouth Medical School
|
Department |
Genetics
|
Lab |
Whitfield
|
Street address |
Dartmouth College; HB7400
|
City |
Hanover |
State/province |
NH |
ZIP/Postal code |
03755 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (2) |
GSE17914 |
IL13 and CCL2 Drive Disease in an Inflammatory Subset of Scleroderma [cGVHD] |
GSE24410 |
IL13 and CCL2 Drive Disease in an Inflammatory Subset of Scleroderma |
|