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Sample GSM4474903 Query DataSets for GSM4474903
Status Public on Sep 26, 2022
Title SLE18 mDC
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: mDC
date measured: 13.11.15
age: 30
Sex: F
sex (1 vs 2): 2
sledai-2k: 8
antidna(ria)antibody titer(u/ml:<6): 24
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 0
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -0.511497
ETG10_236652 0.4497714
ETG10_234183 0.38190365
ETG10_195139 0.5029564
ETG10_13482 0.50579834
ETG09_48764 0.49587727
ETG09_35454 0.52605486
ETG09_205211 0.5398979
ETG08_142674 0.52190876
ETG07_105829 0.7025466
ETG05_66023 0.16618204
ETG05_36762 0.1866641
ETG04_27747 0.4886675
ETG02_36680 0.49199677
ERCC-00171_229 0.4531541
ERCC-00170_100 -0.11207533
ERCC-00168_248 0.5202279
ERCC-00165_60 0.520071
ERCC-00164_60 0.50928307
ERCC-00163_81 0.55164814

Total number of rows: 50739

Table truncated, full table size 1213 Kbytes.

Supplementary file Size Download File type/resource
GSM4474903_US91700289_253949412043_S01_GE1_107_Sep09_2_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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