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Sample GSM4474899 Query DataSets for GSM4474899
Status Public on Sep 26, 2022
Title HC34 mDC
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: mDC
date measured: 13.11.15
age: 33
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.4412408
ETG10_236652 0.19216824
ETG10_234183 0.07362509
ETG10_195139 0.23036623
ETG10_13482 0.31565857
ETG09_48764 0.22914457
ETG09_35454 0.23861551
ETG09_205211 0.24468994
ETG08_142674 0.1630187
ETG07_105829 0.19823551
ETG05_66023 -0.11013031
ETG05_36762 0.61177254
ETG04_27747 0.21543789
ETG02_36680 0.2377696
ERCC-00171_229 0.19979572
ERCC-00170_100 0.5929384
ERCC-00168_248 0.22066784
ERCC-00165_60 0.23648882
ERCC-00164_60 0.22163296
ERCC-00163_81 0.2486744

Total number of rows: 50739

Table truncated, full table size 1220 Kbytes.




Supplementary file Size Download File type/resource
GSM4474899_US91700289_253949412043_S01_GE1_107_Sep09_1_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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