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Sample GSM4474893 Query DataSets for GSM4474893
Status Public on Sep 26, 2022
Title HC32 mDC
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: mDC
date measured: 13.11.08
age: 39
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -1.7914538
ETG10_236652 0.04348612
ETG10_234183 -0.14305258
ETG10_195139 0.094932556
ETG10_13482 0.16619682
ETG09_48764 0.18133259
ETG09_35454 0.11753225
ETG09_205211 0.18790817
ETG08_142674 0.042497158
ETG07_105829 -0.024634361
ETG05_66023 0.028035164
ETG05_36762 0.102371216
ETG04_27747 0.045344353
ETG02_36680 0.09516621
ERCC-00171_229 0.10909605
ERCC-00170_100 0.53605175
ERCC-00168_248 0.09927797
ERCC-00165_60 0.112276554
ERCC-00164_60 0.14936686
ERCC-00163_81 0.07684469

Total number of rows: 50739

Table truncated, full table size 1228 Kbytes.

Supplementary file Size Download File type/resource
GSM4474893_US91700289_253949412035_S01_GE1_107_Sep09_1_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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