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Sample GSM447489 Query DataSets for GSM447489
Status Public on Jun 30, 2011
Title 5718 B10D2 -> balb rag2-/- IL14Ra-/- (new)
Sample type RNA
 
Channel 1
Source name B10D2 -> balb rag2-/- IL14Ra-/-
Organism Mus musculus
Characteristics tissue: skin
Treatment protocol Skin harvested from cGVHD mice at 2 weeks post splenocyte transfer.
Growth protocol cGVHD mice generated by splenocyte transfer of B10.D2 into balb/c; syngeneic controls balb/c into balb/c.
Extracted molecule total RNA
Extraction protocol Total RNA isolated using Trizol methods; cleaned up with Qiagen RNeasy mini-columns.
Label Cy3
Label protocol Agilent Linear Amplification Low RNA Input Kit.
 
Channel 2
Source name Universal Mouse Reference
Organism Mus musculus
Characteristics cell line: multiple
Treatment protocol Skin harvested from cGVHD mice at 2 weeks post splenocyte transfer.
Growth protocol cGVHD mice generated by splenocyte transfer of B10.D2 into balb/c; syngeneic controls balb/c into balb/c.
Extracted molecule total RNA
Extraction protocol Total RNA isolated using Trizol methods; cleaned up with Qiagen RNeasy mini-columns.
Label Cy5
Label protocol Agilent Linear Amplification Low RNA Input Kit.
 
 
Hybridization protocol Agilent Protocols and Hybridization Kits.
Scan protocol GenePix 400B scanner (Axon Instruments).
Description Universal Mouse Reference RNA (Stratagene).
Data processing Feature extraction with GenePix 5.1 software.
 
Submission date Sep 01, 2009
Last update date Jun 30, 2011
Contact name Jennifer L Sargent
E-mail(s) jennifer.sargent@gmail.com
Organization name Dartmouth Medical School
Department Genetics
Lab Whitfield
Street address Dartmouth College; HB7400
City Hanover
State/province NH
ZIP/Postal code 03755
Country USA
 
Platform ID GPL7202
Series (2)
GSE17914 IL13 and CCL2 Drive Disease in an Inflammatory Subset of Scleroderma [cGVHD]
GSE24410 IL13 and CCL2 Drive Disease in an Inflammatory Subset of Scleroderma

Data table header descriptions
ID_REF
VALUE Lowess-normalized log2 ratio (test/reference)

Data table
ID_REF VALUE
A_51_P100021 0
A_51_P100034 -.979
A_51_P100052 0
A_51_P100063 -2.359
A_51_P100084 -.322
A_51_P100099 .11
A_51_P100155 .503
A_51_P100174 2.772
A_51_P100181 -.53
A_51_P100218 0
A_51_P100227 .861
A_51_P100238 0
A_51_P100246 .163
A_51_P100289 .685
A_51_P100298 -.342
A_51_P100309 0
A_51_P100327 -2.276
A_51_P100347 0
A_51_P100379 .621
A_51_P100428 -.009

Total number of rows: 41174

Table truncated, full table size 707 Kbytes.




Supplementary file Size Download File type/resource
GSM447489.gpr.gz 5.4 Mb (ftp)(http) GPR
Processed data included within Sample table

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