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Sample GSM4474889 Query DataSets for GSM4474889
Status Public on Sep 26, 2022
Title SLE64-pre mDC
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: mDC
date measured: 13.11.01
age: 55
Sex: F
sex (1 vs 2): 2
sledai-2k: 7
antidna(ria)antibody titer(u/ml:<6): 165
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 0
pre / post: pre
untreat / treat: untreat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -1.0447183
ETG10_236652 -0.22598648
ETG10_234183 -0.25186777
ETG10_195139 0.010512829
ETG10_13482 -0.26396275
ETG09_48764 0.19471788
ETG09_35454 -0.17558193
ETG09_205211 -0.114851
ETG08_142674 -0.10379267
ETG07_105829 -0.4054656
ETG05_66023 0.004962444
ETG05_36762 -0.36609268
ETG04_27747 -0.19988585
ETG02_36680 -0.20596027
ERCC-00171_229 -0.14471674
ERCC-00170_100 -0.7768841
ERCC-00168_248 -0.13414574
ERCC-00165_60 -0.09251642
ERCC-00164_60 -0.16491413
ERCC-00163_81 -0.16781473

Total number of rows: 50739

Table truncated, full table size 1229 Kbytes.

Supplementary file Size Download File type/resource
GSM4474889_US91700289_253949412034_S01_GE1_107_Sep09_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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