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Sample GSM4474883 Query DataSets for GSM4474883
Status Public on Sep 26, 2022
Title HC12 mDC
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: mDC
date measured: 13.11.01
age: 40
Sex: M
sex (1 vs 2): 1
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -1.1066284
ETG10_236652 0.083498
ETG10_234183 -0.00815773
ETG10_195139 0.11151886
ETG10_13482 -0.056911945
ETG09_48764 0.09869194
ETG09_35454 0.10950899
ETG09_205211 0.11525822
ETG08_142674 0.0645566
ETG07_105829 -0.0487442
ETG05_66023 -0.065834045
ETG05_36762 0.038820744
ETG04_27747 0.4098301
ETG02_36680 0.10819721
ERCC-00171_229 0.19300461
ERCC-00170_100 -0.12983036
ERCC-00168_248 0.08358097
ERCC-00165_60 0.13945103
ERCC-00164_60 0.15075731
ERCC-00163_81 0.033055305

Total number of rows: 50739

Table truncated, full table size 1227 Kbytes.

Supplementary file Size Download File type/resource
GSM4474883_US91700289_253949412034_S01_GE1_107_Sep09_1_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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