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Sample GSM4474866 Query DataSets for GSM4474866
Status Public on Sep 26, 2022
Title SLE47-post Tcell
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: Tcell
date measured: 13.10.09
age: 21
Sex: F
sex (1 vs 2): 2
sledai-2k: 0
antidna(ria)antibody titer(u/ml:<6): 3
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 0
pre / post: post
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.37962103
ETG10_236652 0.17932796
ETG10_234183 0.3580799
ETG10_195139 0.17497444
ETG10_13482 0.098946095
ETG09_48764 0.13492203
ETG09_35454 0.17562294
ETG09_205211 0.20787382
ETG08_142674 0.10598326
ETG07_105829 0.14290762
ETG05_66023 0.3290577
ETG05_36762 -0.0849576
ETG04_27747 0.19523191
ETG02_36680 0.18534184
ERCC-00171_229 0.16230106
ERCC-00170_100 -0.44037914
ERCC-00168_248 0.10264397
ERCC-00165_60 0.17473507
ERCC-00164_60 0.16922474
ERCC-00163_81 0.22428036

Total number of rows: 50739

Table truncated, full table size 1216 Kbytes.

Supplementary file Size Download File type/resource
GSM4474866_US91700289_253949411988_S01_GE1_107_Sep09_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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