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Sample GSM4474865 Query DataSets for GSM4474865
Status Public on Sep 26, 2022
Title SLE47-pre Tcell
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: Tcell
date measured: 13.10.09
age: 21
Sex: F
sex (1 vs 2): 2
sledai-2k: 10
antidna(ria)antibody titer(u/ml:<6): 8
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 0
pre / post: pre
untreat / treat: untreat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.28098583
ETG10_236652 0.06919861
ETG10_234183 0.44822693
ETG10_195139 0.04342985
ETG10_13482 0.068291664
ETG09_48764 -0.009643555
ETG09_35454 0.012511253
ETG09_205211 0.014063835
ETG08_142674 0.22605228
ETG07_105829 0.29565144
ETG05_66023 0.07190561
ETG05_36762 -0.20712471
ETG04_27747 0.25254393
ETG02_36680 0.009279728
ERCC-00171_229 0.082262516
ERCC-00170_100 -0.7989321
ERCC-00168_248 0.06421518
ERCC-00165_60 0.009748459
ERCC-00164_60 0.037629128
ERCC-00163_81 0.15694332

Total number of rows: 50739

Table truncated, full table size 1227 Kbytes.

Supplementary file Size Download File type/resource
GSM4474865_US91700289_253949411988_S01_GE1_107_Sep09_2_2.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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