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Sample GSM4474844 Query DataSets for GSM4474844
Status Public on Sep 26, 2022
Title HC34 monocyte
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: monocyte
date measured: 13.09.19
age: 33
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.004381657
ETG10_236652 -0.050406933
ETG10_234183 -0.16281843
ETG10_195139 0.029407978
ETG10_13482 -0.15190029
ETG09_48764 0.013850212
ETG09_35454 0.024282455
ETG09_205211 0.001094818
ETG08_142674 -0.048591137
ETG07_105829 -0.18339825
ETG05_66023 0.19440651
ETG05_36762 -0.24927235
ETG04_27747 -0.083413124
ETG02_36680 -0.005291462
ERCC-00171_229 -0.026479721
ERCC-00170_100 0.1439991
ERCC-00168_248 -0.036085606
ERCC-00165_60 0.026927471
ERCC-00164_60 0.023004532
ERCC-00163_81 0.012058258

Total number of rows: 50739

Table truncated, full table size 1228 Kbytes.




Supplementary file Size Download File type/resource
GSM4474844_US91700289_253949411961_S01_GE1_107_Sep09_1_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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