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Sample GSM4474843 Query DataSets for GSM4474843
Status Public on Sep 26, 2022
Title SLE71-post pDC
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: pDC
date measured: 13.08.22
age: 26
Sex: F
sex (1 vs 2): 2
sledai-2k: 4
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 1
neurologic disorder (0: Negative, 1: Positive): 0
pre / post: post
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -1.7332625
ETG10_236652 -1.0050244
ETG10_234183 -0.87615585
ETG10_195139 -0.67175484
ETG10_13482 -1.110712
ETG09_48764 -0.85172176
ETG09_35454 -0.9537802
ETG09_205211 -0.96285915
ETG08_142674 -1.1154847
ETG07_105829 -0.92581844
ETG05_66023 -0.9309654
ETG05_36762 -1.194078
ETG04_27747 -0.99815655
ETG02_36680 -0.94585705
ERCC-00171_229 -0.8524065
ERCC-00170_100 0.12752247
ERCC-00168_248 -0.92835426
ERCC-00165_60 -0.9193349
ERCC-00164_60 -0.95968246
ERCC-00163_81 -0.90881777

Total number of rows: 50739

Table truncated, full table size 1222 Kbytes.

Supplementary file Size Download File type/resource
GSM4474843_US91700289_253949411969_S01_GE1_107_Sep09_2_2.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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