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Sample GSM4474836 Query DataSets for GSM4474836
Status Public on Sep 26, 2022
Title SLE39 monocyte
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: monocyte
date measured: 13.08.16
age: 42
Sex: F
sex (1 vs 2): 2
sledai-2k: 0
antidna(ria)antibody titer(u/ml:<6): 4
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 0
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 1.0312476
ETG10_236652 0.6386318
ETG10_234183 0.71089554
ETG10_195139 0.73104477
ETG10_13482 0.59954834
ETG09_48764 0.7210417
ETG09_35454 0.742342
ETG09_205211 0.74582195
ETG08_142674 0.67558384
ETG07_105829 0.5160351
ETG05_66023 0.7494583
ETG05_36762 0.36549473
ETG04_27747 0.7684283
ETG02_36680 0.78001356
ERCC-00171_229 0.7229867
ERCC-00170_100 0.417058
ERCC-00168_248 0.7238884
ERCC-00165_60 0.7546339
ERCC-00164_60 0.7419882
ERCC-00163_81 0.65522623

Total number of rows: 50739

Table truncated, full table size 1199 Kbytes.




Supplementary file Size Download File type/resource
GSM4474836_US91700289_253949412015_S01_GE1_107_Sep09_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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