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Sample GSM4474826 Query DataSets for GSM4474826
Status Public on Sep 26, 2022
Title SLE45 monocyte
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: monocyte
date measured: 13.08.09
age: 46
Sex: F
sex (1 vs 2): 2
sledai-2k: 0
antidna(ria)antibody titer(u/ml:<6): 2
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 1
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.58036375
ETG10_236652 0.12744856
ETG10_234183 0.36190176
ETG10_195139 0.08824825
ETG10_13482 0.021496773
ETG09_48764 0.013776779
ETG09_35454 0.05429411
ETG09_205211 0.13606739
ETG08_142674 0.26376724
ETG07_105829 3.2519321
ETG05_66023 0.5797167
ETG05_36762 0.15417147
ETG04_27747 0.18348885
ETG02_36680 0.03905773
ERCC-00171_229 0.060624123
ERCC-00170_100 1.0325894
ERCC-00168_248 0.16011238
ERCC-00165_60 0.0832634
ERCC-00164_60 0.07329655
ERCC-00163_81 0.20422125

Total number of rows: 50739

Table truncated, full table size 1219 Kbytes.




Supplementary file Size Download File type/resource
GSM4474826_US91700289_253949412014_S01_GE1_107_Sep09_2_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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