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Sample GSM4474806 Query DataSets for GSM4474806
Status Public on Sep 26, 2022
Title HC15 PBMC
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: PBMC
date measured: 13.07.26
age: 34
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -0.06382465
ETG10_236652 -0.3834281
ETG10_234183 -0.51043606
ETG10_195139 -0.3303156
ETG10_13482 -0.4721284
ETG09_48764 -0.35809803
ETG09_35454 -0.3426876
ETG09_205211 -0.3295622
ETG08_142674 -0.18349838
ETG07_105829 -0.4264121
ETG05_66023 -0.28686428
ETG05_36762 -0.3291855
ETG04_27747 -0.44214106
ETG02_36680 -0.34201336
ERCC-00171_229 -0.2833147
ERCC-00170_100 -0.23658705
ERCC-00168_248 -0.33780622
ERCC-00165_60 -0.33464432
ERCC-00164_60 -0.29828978
ERCC-00163_81 -0.25121117

Total number of rows: 50739

Table truncated, full table size 1228 Kbytes.

Supplementary file Size Download File type/resource
GSM4474806_US91700289_253949411965_S01_GE1_107_Sep09_1_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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