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Sample GSM4474805 Query DataSets for GSM4474805
Status Public on Sep 26, 2022
Title SLE45 mDC
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: mDC
date measured: 13.07.18
age: 46
Sex: F
sex (1 vs 2): 2
sledai-2k: 0
antidna(ria)antibody titer(u/ml:<6): 2
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 1
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -1.228929
ETG10_236652 -0.497447
ETG10_234183 -0.14337254
ETG10_195139 -0.41204977
ETG10_13482 -0.6229491
ETG09_48764 -0.49126244
ETG09_35454 -0.44965458
ETG09_205211 -0.4554038
ETG08_142674 -0.5176554
ETG07_105829 -0.61301327
ETG05_66023 -0.5534544
ETG05_36762 -0.41666508
ETG04_27747 -0.55354834
ETG02_36680 -0.43816328
ERCC-00171_229 -0.35305882
ERCC-00170_100 0.45117855
ERCC-00168_248 -0.36539078
ERCC-00165_60 -0.4514246
ERCC-00164_60 -0.40832615
ERCC-00163_81 -0.46600914

Total number of rows: 50739

Table truncated, full table size 1228 Kbytes.




Supplementary file Size Download File type/resource
GSM4474805_US91700289_253949411957_S01_GE1_107_Sep09_2_4.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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