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Sample GSM4474804 Query DataSets for GSM4474804
Status Public on Sep 26, 2022
Title SLE24 mDC
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: mDC
date measured: 13.07.18
age: 23
Sex: F
sex (1 vs 2): 2
sledai-2k: 6
antidna(ria)antibody titer(u/ml:<6): 15
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 2
neurologic disorder (0: Negative, 1: Positive): 0
untreat / treat: treat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.07810259
ETG10_236652 -0.82651615
ETG10_234183 -0.96839714
ETG10_195139 -0.81072235
ETG10_13482 -0.8844099
ETG09_48764 -0.80540466
ETG09_35454 -0.82011986
ETG09_205211 -0.81540823
ETG08_142674 -0.73442984
ETG07_105829 -0.9879761
ETG05_66023 -0.5322037
ETG05_36762 -0.82027817
ETG04_27747 -0.80285263
ETG02_36680 -0.7055321
ERCC-00171_229 -0.81822395
ERCC-00170_100 -0.6913662
ERCC-00168_248 -0.68412924
ERCC-00165_60 -0.8150735
ERCC-00164_60 -0.8117504
ERCC-00163_81 -0.75888443

Total number of rows: 50739

Table truncated, full table size 1224 Kbytes.

Supplementary file Size Download File type/resource
GSM4474804_US91700289_253949411957_S01_GE1_107_Sep09_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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