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Sample GSM4474785 Query DataSets for GSM4474785
Status Public on Sep 26, 2022
Title SLE43 Tcell
Sample type RNA
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: SLE
cell type: Tcell
date measured: 13.07.03
age: 65
Sex: F
sex (1 vs 2): 2
sledai-2k: 10
antidna(ria)antibody titer(u/ml:<6): 4
renal disorder (0: Negative, 1: Positive, 2: Biopsy-proven): 0
neurologic disorder (0: Negative, 1: Positive): 1
pre / post: pre
untreat / treat: untreat
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.006737232
ETG10_236652 -0.3945055
ETG10_234183 -0.081820965
ETG10_195139 -0.4680977
ETG10_13482 -0.4843545
ETG09_48764 -0.4803095
ETG09_35454 -0.45466518
ETG09_205211 -0.35308266
ETG08_142674 -0.41287518
ETG07_105829 -0.717947
ETG05_66023 -0.74385643
ETG05_36762 -0.38133192
ETG04_27747 -0.543561
ETG02_36680 -0.46431828
ERCC-00171_229 -0.3872633
ERCC-00170_100 -0.45528316
ERCC-00168_248 -0.47493744
ERCC-00165_60 -0.47079277
ERCC-00164_60 -0.3592105
ERCC-00163_81 -0.5171056

Total number of rows: 50739

Table truncated, full table size 1222 Kbytes.

Supplementary file Size Download File type/resource
GSM4474785_US91700289_253949411984_S01_GE1_107_Sep09_1_4.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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