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Sample GSM4474775 Query DataSets for GSM4474775
Status Public on Sep 26, 2022
Title HC14 memory-B
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics diagnosis: Healthy control
cell type: memory-B
date measured: 13.06.26
age: 38
Sex: F
sex (1 vs 2): 2
Treatment protocol Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
Label Cy3
Label protocol Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
 
Hybridization protocol Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
Scan protocol Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
Data processing Data processing and trimming were performed with GeneSpring ver. 12.6.1.
 
Submission date Apr 13, 2020
Last update date Sep 26, 2022
Contact name Kenji Okuma
E-mail(s) kenji.okuma@astellas.com
Phone +81-75-366-7462
Organization name Astellas Pharma Inc.
Street address 53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
City Kyoto
State/province Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platform ID GPL17077
Series (1)
GSE148601 mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.13388252
ETG10_236652 -0.32895994
ETG10_234183 -0.44955778
ETG10_195139 -0.48871088
ETG10_13482 -0.5536022
ETG09_48764 -0.47919846
ETG09_35454 -0.45714664
ETG09_205211 -0.47560024
ETG08_142674 -0.58360004
ETG07_105829 -0.68621063
ETG05_66023 -0.8301153
ETG05_36762 -0.82997036
ETG04_27747 -0.55398035
ETG02_36680 -0.4663067
ERCC-00171_229 -0.4505515
ERCC-00170_100 -0.4079275
ERCC-00168_248 -0.4593482
ERCC-00165_60 -0.45769453
ERCC-00164_60 -0.35612202
ERCC-00163_81 -0.4926138

Total number of rows: 50739

Table truncated, full table size 1222 Kbytes.




Supplementary file Size Download File type/resource
GSM4474775_US91700289_253949411972_S01_GE1_107_Sep09_1_2.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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