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Sample GSM447344 Query DataSets for GSM447344
Status Public on Dec 15, 2012
Title THP-1_LPS+TGF_1
Sample type RNA
 
Source name THP-1 monocytes, LPS+TGF, 24hrs
Organism Homo sapiens
Characteristics cell line: THP-1 monocytic cell line
agent (stimulus): LPS+TGF
Treatment protocol THP-1 cells were stimulated with LPS (100ng/mL) or TGFβ1 (1ng/mL) or LPS and TGFβ1 for 24 hrs and kept in an CO2 injecting incubator. As a control PBS was added to the cells.
Growth protocol THP-1 cells were cultured in RPMI-1640 medium containing 50mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 5% FBS. Monocytes, in a density of 2*10^6 cells/4 mL were starved O/N prior to stimulation in medium containing 0.1% FCS.
Extracted molecule total RNA
Extraction protocol RNA of stimulated monocytes was isolated using Tripure reagent (Roche) according the manufacturer’s protocol. High quality RNA was obtained with NucleoSpin RNA II kits (Machery-Nagel) inclusive DNase treatment
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kits were used for cDNA and cRNA synthesis (Ambion) according the manufacturer’s protocol
 
Hybridization protocol According to beadchip array manufacturer's protocol
Scan protocol According to beadchip array manufacturer's protocol
Description 4223164108_E
Data processing Gene expression values of the chips were normalized in BeadStudio (version 3.2 Illumina)
 
Submission date Sep 01, 2009
Last update date Dec 15, 2012
Contact name Karlijn van Keulen
E-mail(s) j.k.vankeulen@umcutrecht.nl
Phone 0031307557155
Organization name University Medical Center Utrecht
Department division heart and lungs
Lab Experimental Cardiology
Street address Heidelberglaan 100, G02.523
City Utrecht
ZIP/Postal code 3584 CX
Country Netherlands
 
Platform ID GPL6883
Series (1)
GSE17909 Effect of simultaneous activation of TGFbeta and TLR4 pathway in human monocytes

Data table header descriptions
ID_REF
VALUE rank invariant normalized after background subtraction
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 163.7285 0
ILMN_1660305 420.5832 0
ILMN_1762337 -0.7581709 0.75387
ILMN_2055271 34.88484 0.02321981
ILMN_1814316 4.829249 0.5650155
ILMN_2359168 7.920638 0.4551083
ILMN_1731507 2.6118 0.6501548
ILMN_1787689 14.9348 0.2724458
ILMN_1745607 188.6123 0
ILMN_2136495 12.3042 0.3343653
ILMN_1668111 -8.2643 0.9071208
ILMN_2295559 14.09443 0.2941177
ILMN_1735045 67.7314 0
ILMN_1680754 -14.32325 0.9705882
ILMN_2375184 -5.207882 0.8513932
ILMN_1659452 22.54619 0.125387
ILMN_1767388 4.744395 0.5712074
ILMN_1675204 -19.01292 0.9876161
ILMN_1673870 28.16645 0.0619195
ILMN_1755321 76.37871 0

Total number of rows: 24518

Table truncated, full table size 672 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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