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Sample GSM447322 Query DataSets for GSM447322
Status Public on Dec 15, 2012
Title THP-1_PBS_3
Sample type RNA
 
Source name THP-1 monocytes, PBS, 24hrs
Organism Homo sapiens
Characteristics cell line: THP-1 monocytic cell line
agent (stimulus): PBS
Treatment protocol THP-1 cells were stimulated with LPS (100ng/mL) or TGFβ1 (1ng/mL) or LPS and TGFβ1 for 24 hrs and kept in an CO2 injecting incubator. As a control PBS was added to the cells.
Growth protocol THP-1 cells were cultured in RPMI-1640 medium containing 50mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, and 5% FBS. Monocytes, in a density of 2*10^6 cells/4 mL were starved O/N prior to stimulation in medium containing 0.1% FCS.
Extracted molecule total RNA
Extraction protocol RNA of stimulated monocytes was isolated using Tripure reagent (Roche) according the manufacturer’s protocol. High quality RNA was obtained with NucleoSpin RNA II kits (Machery-Nagel) inclusive DNase treatment
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kits were used for cDNA and cRNA synthesis (Ambion) according the manufacturer’s protocol
 
Hybridization protocol According to beadchip array manufacturer's protocol
Scan protocol According to beadchip array manufacturer's protocol
Description 4223164097_A
Data processing Gene expression values of the chips were normalized in BeadStudio (version 3.2 Illumina)
 
Submission date Sep 01, 2009
Last update date Dec 15, 2012
Contact name Karlijn van Keulen
E-mail(s) j.k.vankeulen@umcutrecht.nl
Phone 0031307557155
Organization name University Medical Center Utrecht
Department division heart and lungs
Lab Experimental Cardiology
Street address Heidelberglaan 100, G02.523
City Utrecht
ZIP/Postal code 3584 CX
Country Netherlands
 
Platform ID GPL6883
Series (1)
GSE17909 Effect of simultaneous activation of TGFbeta and TLR4 pathway in human monocytes

Data table header descriptions
ID_REF
VALUE rank invariant normalized after background subtraction
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 79.79164 0
ILMN_1660305 483.6638 0
ILMN_1762337 5.713413 0.523511
ILMN_2055271 42.1912 0
ILMN_1814316 2.274962 0.6661442
ILMN_2359168 9.259363 0.3981191
ILMN_1731507 0.7203705 0.7319749
ILMN_1787689 7.151275 0.468652
ILMN_1745607 27.61929 0.04388715
ILMN_2136495 2.508497 0.6598746
ILMN_1668111 -7.121941 0.9278997
ILMN_2295559 8.978619 0.4090909
ILMN_1735045 106.3475 0
ILMN_1680754 6.63082 0.4905956
ILMN_2375184 -2.568766 0.8338558
ILMN_1659452 20.53992 0.130094
ILMN_1767388 23.15557 0.0846395
ILMN_1675204 4.181057 0.6097178
ILMN_1673870 20.85185 0.1253919
ILMN_1755321 93.26707 0

Total number of rows: 24518

Table truncated, full table size 668 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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