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Sample GSM446986 Query DataSets for GSM446986
Status Public on Aug 15, 2010
Title BMP-2 treated for 1 h at 25ng/ml rep1
Sample type RNA
 
Source name Mouse Embryonic stem cells(E14Tg2A.4)
Organism Mus musculus
Characteristics cell line: E14Tg2A.4
Biomaterial provider MMRRC at university of california, Davis
Extracted molecule total RNA
Extraction protocol USB total RNA extraction kit
Label biotin
Label protocol biotinylated cRNA were prepared from 10ug of total RNA using the standard labeling techniques for affymetrix chips
 
Hybridization protocol 10 ug cRNA were hybridized to the affymetrix MOE 430 2.0 chips
Scan protocol genechips were scanned using the Hewiett-Packard genearray scanner
Description E14Tg2A.4 cells were treated with BMP-2 at 25ng/ml for 1 hour
Data processing MAS 5.0
 
Submission date Aug 31, 2009
Last update date Sep 01, 2009
Contact name Qiaoran Xi
E-mail(s) xiq@mskcc.org
Organization name MSKCC
Department Cancer biology and genetics
Lab Joan Massague
Street address 1275, York ave.
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL1261
Series (1)
GSE17896 Mouse Embryonic Stem Cell E14Tg2A.4 BMP2 Responses

Data table header descriptions
ID_REF
VALUE Mas5-caculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if transcript was present(p), absent(A), or marginal(M), or nor call(NC)
DETECTION P-VALUE P-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 284.451 P 0.000340305
AFFX-BioB-M_at 495.211 P 6.02111e-05
AFFX-BioB-3_at 268.56 P 6.02111e-05
AFFX-BioC-5_at 1057.51 P 7.00668e-05
AFFX-BioC-3_at 1003.39 P 4.42873e-05
AFFX-BioDn-5_at 1949.52 P 4.42873e-05
AFFX-BioDn-3_at 4438.39 P 0.000296708
AFFX-CreX-5_at 13658.6 P 5.16732e-05
AFFX-CreX-3_at 14853 P 4.42873e-05
AFFX-DapX-5_at 16.3068 A 0.131361
AFFX-DapX-M_at 17.596 A 0.275146
AFFX-DapX-3_at 4.95885 A 0.772364
AFFX-LysX-5_at 3.96602 A 0.58862
AFFX-LysX-M_at 15.3181 A 0.749204
AFFX-LysX-3_at 4.96455 A 0.574038
AFFX-PheX-5_at 0.981871 A 0.971543
AFFX-PheX-M_at 1.6434 A 0.960339
AFFX-PheX-3_at 28.2306 A 0.275146
AFFX-ThrX-5_at 19.7524 A 0.41138
AFFX-ThrX-M_at 1.76813 A 0.749223

Total number of rows: 45101

Table truncated, full table size 1377 Kbytes.




Supplementary file Size Download File type/resource
GSM446986.CEL.gz 6.3 Mb (ftp)(http) CEL
GSM446986.CHP.gz 11.5 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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