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Sample GSM4453132 Query DataSets for GSM4453132
Status Public on Jun 08, 2020
Title P3_Jmjd1aKO_rep1
Sample type SRA
 
Source name P3_Jmjd1aKO_postinatal male germ cells
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype: Vasa-Cre; Jmjd1a-/-; Jmjd1b-/+
age: P3
tissue: testis
cell type: Postnatal male germ cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using All Prep DNA/RNA Micro Kit (Qiagen). cDNA library was prepared from total RNA using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Sequenced reads were trimmed for adaptor sequence, then mapped to mm10 whole genome using TopHat2 v2.1.1 with default parameters.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were estimated using Cufflinks v2.1.1.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
 
Submission date Apr 03, 2020
Last update date Jun 09, 2020
Contact name Ryo Maeda
E-mail(s) rmaeda@fbs.osaka-u.ac.jp
Organization name Osaka University
Street address Yamadaoka 1-3
City Suita
ZIP/Postal code 565-0871
Country Japan
 
Platform ID GPL13112
Series (2)
GSE148053 Total RNA sequencing of postnatal male germ cells
GSE148055 RNA-seq and H3K9me2 ChIP-seq of postnatal male germ cells
Relations
BioSample SAMN14533676
SRA SRX8053157

Supplementary file Size Download File type/resource
GSM4453132_P3_Jmjd1aKO_rep1_RPKM.txt.gz 150.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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