|
| Status |
Public on Apr 03, 2020 |
| Title |
CT05 |
| Sample type |
SRA |
| |
|
| Source name |
Second antennal segment
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Canton-S
tissue: Second antennal segment
age: 5 days old
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were anesthetised on ice, their second antennal segments (which contain the flies' Johnston's organs) were dissected, collected in Lysis Buffer (containing 1% beta-mercaptoethanol, as provided in the Qiagen Rneasy Mini kit) and subsequently placed in storage at -80 degrees Celsius. Samples were removed from the freezer for RNA extractions: These were conducted according to the Qiagen RNeasy Mini kit protocol.
Sample libraries were prepared with a Nextera XT DNA Library Preparation kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
processed_data.xlsx
|
| Data processing |
Sequenced reads were mapped to the dm6 Drosophila melanogaster reference genome using Partek Flow software.
Read counts were produced using HTSeq from the bam files generated by Partek Flow.
Normalization of read counts and subsequent statistical analyses were conducted with DESeq2.
Genome_build: dm6
Supplementary_files_format_and_content: The processed_data.xlsx file includes the DESeq2-normalized counts for all samples (columns labeled ct01 to ct18), as well as the results of the statistical comparisons between them.
|
| |
|
| Submission date |
Apr 02, 2020 |
| Last update date |
Apr 05, 2020 |
| Contact name |
Joerg Albert |
| E-mail(s) |
joerg.albert@ucl.ac.uk
|
| Organization name |
University College London
|
| Department |
Ear Institute
|
| Lab |
Albert Lab
|
| Street address |
332 Grays Inn Rd
|
| City |
London |
| ZIP/Postal code |
WC1X 8EE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE148023 |
Homeostatic maintenance and age-related functional decline in the Drosophila ear |
|
| Relations |
| BioSample |
SAMN14530222 |
| SRA |
SRX8049137 |
