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Sample GSM4446330 Query DataSets for GSM4446330
Status Public on Dec 21, 2021
Title TB14-4
Sample type RNA
Source name AML cell line expressing Trib1 and Bcl11a
Organism Mus musculus
Characteristics cell line: Trib1- and Bcl11a-expressing murine AML cells
Treatment protocol No treatment was provided.
Growth protocol Trib1, TB13 and TB14 cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
Description Gene expression data from mouse AML
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Mar 30, 2020
Last update date Dec 21, 2021
Contact name Takuro Nakamura
Phone 81-3-3570-0462
Organization name Japanese Foundation for Cancer Research
Department The Cancer Institute
Lab Carcinogenesis
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
Platform ID GPL11180
Series (2)
GSE147797 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1 [array]
GSE147798 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 61.921795
AFFX-BioB-M_at 103.40517
AFFX-BioB-3_at 91.46922
AFFX-BioC-5_at 199.6964
AFFX-BioC-3_at 238.10608
AFFX-BioDn-5_at 391.33875
AFFX-BioDn-3_at 991.32916
AFFX-CreX-5_at 2261.4556
AFFX-CreX-3_at 2618.7712
AFFX-DapX-5_at 116.551926
AFFX-DapX-M_at 246.12296
AFFX-DapX-3_at 234.22485
AFFX-LysX-5_at 9.8688755
AFFX-LysX-M_at 13.995947
AFFX-LysX-3_at 27.59614
AFFX-PheX-5_at 21.312847
AFFX-PheX-M_at 23.842842
AFFX-PheX-3_at 35.6175
AFFX-ThrX-5_at 29.230757
AFFX-ThrX-M_at 47.280357

Total number of rows: 45141

Table truncated, full table size 1061 Kbytes.

Supplementary file Size Download File type/resource 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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