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Sample GSM4446328 Query DataSets for GSM4446328
Status Public on Dec 21, 2021
Title TB14-2
Sample type RNA
 
Source name AML cell line expressing Trib1 and Bcl11a
Organism Mus musculus
Characteristics cell line: Trib1- and Bcl11a-expressing murine AML cells
Treatment protocol No treatment was provided.
Growth protocol Trib1, TB13 and TB14 cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
Description Gene expression data from mouse AML
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Mar 30, 2020
Last update date Dec 21, 2021
Contact name Takuro Nakamura
E-mail(s) takuro-ind@umin.net
Phone 81-3-3570-0462
Organization name Japanese Foundation for Cancer Research
Department The Cancer Institute
Lab Carcinogenesis
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
 
Platform ID GPL11180
Series (2)
GSE147797 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1 [array]
GSE147798 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 56.759644
AFFX-BioB-M_at 104.269066
AFFX-BioB-3_at 91.95544
AFFX-BioC-5_at 195.42017
AFFX-BioC-3_at 243.69376
AFFX-BioDn-5_at 395.30475
AFFX-BioDn-3_at 1016.10004
AFFX-CreX-5_at 2305.4546
AFFX-CreX-3_at 2619.0032
AFFX-DapX-5_at 101.02219
AFFX-DapX-M_at 213.23198
AFFX-DapX-3_at 193.54288
AFFX-LysX-5_at 10.349867
AFFX-LysX-M_at 14.193755
AFFX-LysX-3_at 23.027384
AFFX-PheX-5_at 20.214043
AFFX-PheX-M_at 23.416843
AFFX-PheX-3_at 34.89183
AFFX-ThrX-5_at 25.904526
AFFX-ThrX-M_at 32.15597

Total number of rows: 45141

Table truncated, full table size 1061 Kbytes.




Supplementary file Size Download File type/resource
GSM4446328_TB14-2.ga.cel.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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