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Sample GSM4446326 Query DataSets for GSM4446326
Status Public on Dec 21, 2021
Title TB13-4
Sample type RNA
 
Source name AML cell line expressing Trib1 and Bcl11a
Organism Mus musculus
Characteristics cell line: Trib1- and Bcl11a-expressing murine AML cells
Treatment protocol No treatment was provided.
Growth protocol Trib1, TB13 and TB14 cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
Description Gene expression data from mouse AML
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Mar 30, 2020
Last update date Dec 21, 2021
Contact name Takuro Nakamura
E-mail(s) takuro-ind@umin.net
Phone 81-3-3570-0462
Organization name Japanese Foundation for Cancer Research
Department The Cancer Institute
Lab Carcinogenesis
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
 
Platform ID GPL11180
Series (2)
GSE147797 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1 [array]
GSE147798 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 26.807087
AFFX-BioB-M_at 29.395218
AFFX-BioB-3_at 37.406853
AFFX-BioC-5_at 73.63716
AFFX-BioC-3_at 99.32587
AFFX-BioDn-5_at 186.09778
AFFX-BioDn-3_at 754.11334
AFFX-CreX-5_at 2241.8826
AFFX-CreX-3_at 2591.074
AFFX-DapX-5_at 50.32735
AFFX-DapX-M_at 102.734055
AFFX-DapX-3_at 124.550545
AFFX-LysX-5_at 9.137605
AFFX-LysX-M_at 11.80285
AFFX-LysX-3_at 15.820446
AFFX-PheX-5_at 17.97757
AFFX-PheX-M_at 17.234451
AFFX-PheX-3_at 25.455057
AFFX-ThrX-5_at 23.490734
AFFX-ThrX-M_at 23.592993

Total number of rows: 45141

Table truncated, full table size 1061 Kbytes.




Supplementary file Size Download File type/resource
GSM4446326_TB13-4.ga.cel.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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