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Sample GSM4446320 Query DataSets for GSM4446320
Status Public on Dec 21, 2021
Title Trib1 2
Sample type RNA
Source name AML cell line expressing Trib1
Organism Mus musculus
Characteristics genotype: Trib1-expressing murine AML cells
Treatment protocol No treatment was provided.
Growth protocol Trib1, TB13 and TB14 cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
Description Gene expression data from mouse AML
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Mar 30, 2020
Last update date Dec 21, 2021
Contact name Takuro Nakamura
Phone 81-3-3570-0462
Organization name Japanese Foundation for Cancer Research
Department The Cancer Institute
Lab Carcinogenesis
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
Platform ID GPL11180
Series (2)
GSE147797 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1 [array]
GSE147798 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 55.60832
AFFX-BioB-M_at 98.271324
AFFX-BioB-3_at 88.320465
AFFX-BioC-5_at 196.37454
AFFX-BioC-3_at 246.91656
AFFX-BioDn-5_at 399.4609
AFFX-BioDn-3_at 1057.5658
AFFX-CreX-5_at 2375.0422
AFFX-CreX-3_at 2716.5042
AFFX-DapX-5_at 104.17702
AFFX-DapX-M_at 244.38712
AFFX-DapX-3_at 233.15996
AFFX-LysX-5_at 10.293043
AFFX-LysX-M_at 13.193143
AFFX-LysX-3_at 27.53113
AFFX-PheX-5_at 21.407267
AFFX-PheX-M_at 24.98318
AFFX-PheX-3_at 35.52239
AFFX-ThrX-5_at 29.01266
AFFX-ThrX-M_at 37.384396

Total number of rows: 45141

Table truncated, full table size 1061 Kbytes.

Supplementary file Size Download File type/resource 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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