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Sample GSM4435314 Query DataSets for GSM4435314
Status Public on Apr 17, 2020
Title WT,GAR--30C-IP rep2
Sample type SRA
 
Source name WT,GAR--30C-IP rep2
Organism Saccharomyces cerevisiae
Characteristics strain: GAR
culture temperature: 30
chip antibody: 8WG16; Covance MMS-126R
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated yeast cells and Rbp1-DNA complexes were isolated with antibody.Associated DNAs were purified by PCR clean-up kit (Axygen)
Libraries were prepared according to Illumina's instructions by Novogene.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model HiSeq X Ten
 
Data processing ChIP-seq reads were aligned to S. cerevisiae genome using bwa version 0.7.17-r1188 with the following configurations…
reads that were marked as duplicates and mapped to multiple locations were removed by samtools version 1.9
read counts per bin were normalized to one million mapped reads (RPM) by deeptools version 3.2.1
Calling peak used MACS2 version 2.1.2
Genome_build: R64-1-1
 
Submission date Mar 26, 2020
Last update date Apr 18, 2020
Contact name Zhan Ding
E-mail(s) dingzhan@cemps.ac.cn
Organization name CAS Center for Excellence in Molecular Plant Science
Street address FengLin 300
City Shanghai
ZIP/Postal code 200032
Country China
 
Platform ID GPL26171
Series (1)
GSE147179 Prp5-Spt8/Spt3 Interaction Mediates a Reciprocal Coupling between Splicing and Transcription
Relations
BioSample SAMN14501496
SRA SRX8036501

Supplementary file Size Download File type/resource
GSM4435314_02-GAR--30C-IP_R2.mLb.clN.bigWig 28.0 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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