|
Status |
Public on Jun 05, 2020 |
Title |
splenic monocytes from B6 mice at 1wk post immunization rep1 |
Sample type |
SRA |
|
|
Source name |
spleen
|
Organism |
Mus musculus |
Characteristics |
tissue: spleen age: 1 week genotype: C57BL/6J (B6, H-2b),
|
Extracted molecule |
total RNA |
Extraction protocol |
Sorted cells were loaded into the Single Cell Chip and processed through the 10X controller for droplet generation, resulting in droplets containing a single cell and bead per droplet Chromium Single Cell 3' RNA-seq libraries were prepared for sequencing using standard 10X Genomics protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
scRNA data from lymph node
|
Data processing |
Standard 10X Genomics CellRanger pipeline Genome_build: mm10 Supplementary_files_format_and_content: for each sample, there are one mtx file with filtered gene expressing UMI counts for each sample; a tsv file containing gene names; a tsv file with cell barcodes.
|
|
|
Submission date |
Mar 26, 2020 |
Last update date |
Jun 05, 2020 |
Contact name |
Wei Chen |
E-mail(s) |
wei.chen@chp.edu
|
Organization name |
Children’s Hospital of Pittsburgh of UPMC
|
Department |
Division of Pulmonary Medicine, Allergy and Immunology, Department of Pediatrics
|
Street address |
4401 Penn Ave., Rangos Research Building, Room 9125
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15224 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE147596 |
Paired immunoglobulin-like receptors mediate innate memory to non-self MHC molecules |
|
Relations |
BioSample |
SAMN14464376 |
SRA |
SRX8008443 |