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Sample GSM4435026 Query DataSets for GSM4435026
Status Public on Jun 05, 2020
Title splenic monocytes from B6 mice at 1wk post immunization rep1
Sample type SRA
 
Source name spleen
Organism Mus musculus
Characteristics tissue: spleen
age: 1 week
genotype: C57BL/6J (B6, H-2b),
Extracted molecule total RNA
Extraction protocol Sorted cells were loaded into the Single Cell Chip and processed through the 10X controller for droplet generation, resulting in droplets containing a single cell and bead per droplet
Chromium Single Cell 3' RNA-seq libraries were prepared for sequencing using standard 10X Genomics protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description scRNA data from lymph node
Data processing Standard 10X Genomics CellRanger pipeline
Genome_build: mm10
Supplementary_files_format_and_content: for each sample, there are one mtx file with filtered gene expressing UMI counts for each sample; a tsv file containing gene names; a tsv file with cell barcodes.
 
Submission date Mar 26, 2020
Last update date Jun 05, 2020
Contact name Wei Chen
E-mail(s) wei.chen@chp.edu
Organization name Children’s Hospital of Pittsburgh of UPMC
Department Division of Pulmonary Medicine, Allergy and Immunology, Department of Pediatrics
Street address 4401 Penn Ave., Rangos Research Building, Room 9125
City Pittsburgh
State/province PA
ZIP/Postal code 15224
Country USA
 
Platform ID GPL19057
Series (1)
GSE147596 Paired immunoglobulin-like receptors mediate innate memory to non-self MHC molecules
Relations
BioSample SAMN14464376
SRA SRX8008443

Supplementary file Size Download File type/resource
GSM4435026_week1_B6_1_barcodes.tsv.gz 18.5 Kb (ftp)(http) TSV
GSM4435026_week1_B6_1_features.tsv.gz 244.7 Kb (ftp)(http) TSV
GSM4435026_week1_B6_1_matrix.mtx.gz 21.4 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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