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Sample GSM4433513 Query DataSets for GSM4433513
Status Public on Aug 19, 2021
Title Nascent_C2plusTplusE2_Daniel
Sample type SRA
 
Source name Immortalized mouse embryonic fibroblast line A, Ezh2 -/- , retroviral pMSCV-Ezh2
Organism Mus musculus
Characteristics cell type: c-myc-immortalized mouse embryonic fibroblasts
genotype: Ezh2 -/- , retroviral pMSCV-Ezh2
fraction: nascent RNA
Growth protocol MEF cells were grown in DMEM supplemented with 10% FCS, 100 mM nonessential amino acids, 2 mM L-glutamine.
Extracted molecule total RNA
Extraction protocol Truseq Stranded Total RNA
Total or nascent RNA was extracted using Trizol reageant following manufacturer's instructions
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Adapter trimming (Trim Galore! 0.4.4 wrapper with default settings)
Discard reads aligning to complete mouse rRNA sequence (Bowtie 2.2.9)
Alignment to mouse genome (Bowtie 2.2.9)
Gene counts (FeatureCounts 1.5.1)
Genome_build: mm10
Supplementary_files_format_and_content: gene_counts.txt files contain gene counts
 
Submission date Mar 26, 2020
Last update date Aug 19, 2021
Contact name Daniel Holoch
E-mail(s) daniel.holoch@curie.fr
Phone 0156246553
Organization name Institut Curie, Centre de Recherche
Department Genetics and Developmental Biology Unit
Lab Raphaƫl Margueron
Street address 26 rue d'Ulm
City Paris
State/province Cedex 05
ZIP/Postal code 75248
Country France
 
Platform ID GPL17021
Series (2)
GSE147563 A cis-acting mechanism mediates trancriptional memory at Polycomb target genes in mammals [RNA-Seq]
GSE147568 A cis-acting mechanism mediates trancriptional memory at Polycomb target genes in mammals
Relations
BioSample SAMN14452171
SRA SRX8005958

Supplementary file Size Download File type/resource
GSM4433513_Nascent_C2plusTplusE2_Daniel_gene_counts.txt.gz 875.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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