|
Status |
Public on Sep 21, 2011 |
Title |
Σ1278b FLO8 overexpressed, biological rep2 |
Sample type |
RNA |
|
|
Source name |
Wild type yeast transformed with muticopy vector carrying FLO8
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: Σ1278b, H-U Moesch genotype: MATα ura3-52 trp1Δ::hisG leu2Δ::hisG his3Δ::hisG plasmid: YEpLac195
|
Treatment protocol |
Yeast cultures grown to early stationary phase, cells were seperated from growth medium, washed and mixed with extraction buffer and phenol/chloroform before being frozen in liquid nitrogen
|
Growth protocol |
Yeast grown in 5ml (test tubes on rotary wheel) synthetic dropout media (YNB) with 2% glucose at 30 degrees Celsius. Total RNA harvested at entry into stationary phase
|
Extracted molecule |
total RNA |
Extraction protocol |
Phenol-Chloroform exctraction with heat treatment (65 degrees Celsius 5 min.). Schmitt et al., 1990, A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res 18: 3091-3092.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Performed according to standard Affymetrix protocol
|
Scan protocol |
GeneChips were scanned using Affymetrix GeneChip® scanner 3000and the Affymetrix GeneChip® Operating Software (GCOS) version 1.4.
|
Description |
Gene expression data of yeast grown till early stationary phase in synthetic dropout media
|
Data processing |
Data normalisation performed with GC-RMA
|
|
|
Submission date |
Aug 19, 2009 |
Last update date |
Sep 23, 2011 |
Contact name |
Michael Christiaan Bester |
E-mail(s) |
bester@sun.ac.za
|
Phone |
+27218084867
|
Organization name |
Institute for Wine Biotechnology
|
Lab |
Yeast Lab
|
Street address |
Victoria street
|
City |
Stellenbosch |
ZIP/Postal code |
7600 |
Country |
South Africa |
|
|
Platform ID |
GPL2529 |
Series (1) |
GSE17716 |
Transcription data from Saccharomyces cerevisiae yeast |
|