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Sample GSM442214 Query DataSets for GSM442214
Status Public on Sep 21, 2011
Title Σ1278b FLO8 overexpressed, biological rep2
Sample type RNA
 
Source name Wild type yeast transformed with muticopy vector carrying FLO8
Organism Saccharomyces cerevisiae
Characteristics strain: Σ1278b, H-U Moesch
genotype: MATα ura3-52 trp1Δ::hisG leu2Δ::hisG his3Δ::hisG
plasmid: YEpLac195
Treatment protocol Yeast cultures grown to early stationary phase, cells were seperated from growth medium, washed and mixed with extraction buffer and phenol/chloroform before being frozen in liquid nitrogen
Growth protocol Yeast grown in 5ml (test tubes on rotary wheel) synthetic dropout media (YNB) with 2% glucose at 30 degrees Celsius. Total RNA harvested at entry into stationary phase
Extracted molecule total RNA
Extraction protocol Phenol-Chloroform exctraction with heat treatment (65 degrees Celsius 5 min.). Schmitt et al., 1990, A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res 18: 3091-3092.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Performed according to standard Affymetrix protocol
Scan protocol GeneChips were scanned using Affymetrix GeneChip® scanner 3000and the Affymetrix GeneChip® Operating Software (GCOS) version 1.4.
Description Gene expression data of yeast grown till early stationary phase in synthetic dropout media
Data processing Data normalisation performed with GC-RMA
 
Submission date Aug 19, 2009
Last update date Sep 23, 2011
Contact name Michael Christiaan Bester
E-mail(s) bester@sun.ac.za
Phone +27218084867
Organization name Institute for Wine Biotechnology
Lab Yeast Lab
Street address Victoria street
City Stellenbosch
ZIP/Postal code 7600
Country South Africa
 
Platform ID GPL2529
Series (1)
GSE17716 Transcription data from Saccharomyces cerevisiae yeast

Data table header descriptions
ID_REF
VALUE GC-RMA signal

Data table
ID_REF VALUE
1769308_at 1054.865921
1769309_at 4.530804969
1769310_at 3.8173014
1769311_at 10307.773
1769312_at 1920.264473
1769313_at 1004.565311
1769314_at 7123.253022
1769315_at 3.967251544
1769316_s_at 3.961088022
1769317_at 1286.863297
1769318_at 3.74715373
1769319_at 5949.623818
1769320_at 649.7389568
1769321_at 3.80164276
1769322_s_at 26.92730259
1769323_at 2672.681596
1769324_at 746.4493004
1769325_at 493.8673006
1769326_at 3.78373107
1769327_at 3.655531242

Total number of rows: 10928

Table truncated, full table size 246 Kbytes.




Supplementary file Size Download File type/resource
GSM442214.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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