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Sample GSM4417010 Query DataSets for GSM4417010
Status Public on Sep 13, 2020
Title Fraction 7#3
Sample type SRA
Source name Fraction 7 B cells
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: spleen
genotype: Bcl6-YFP(KI/+) B1-8hi(KI/+)
cell type: Fraction 7 B cells (mature memory B cells)
Growth protocol Bcl6-YFP B1-8hi naïve B cells were transferred into wild-type CD45.1+ mice. Mice were immunized i.p. (day 0) with NP-CGG/alum and NP-specific IgG1+ donor GC LZ (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4lo), Fr.2 (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4loBcl6-YFPhiCD69hi), Fr.3 (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4loBcl6-YFPhiCD69lo), Fr.5 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPhiEfnb1hi), Fr.6 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPloEfnb1hi), and Fr.7 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPloEfnb1lo) B cells were sorted from pooled splenocytes of 9 recipient mice on day 10.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol Reagent (Invitrogen).
DNA library was constructed using an NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) from total RNA purified from 1~2 × 10^4 sorted cells.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Data processing Read alignment: TopHat2 v2.0.8 with default settings
expression level: Cufflinks v2.1.1 with default settings
Genome_build: mm9
Supplementary_files_format_and_content: pro-mem RNA-seq.txt
Submission date Mar 17, 2020
Last update date Sep 13, 2020
Contact name Takeshi Inoue
Organization name Osaka University
Street address 3-1 Yamadaoka
City Suita-shi
State/province Osaka
ZIP/Postal code 5650871
Country Japan
Platform ID GPL18480
Series (2)
GSE147095 mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center [2]
GSE147096 mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center
BioSample SAMN14390997
SRA SRX7924367

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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