|
| Status |
Public on Sep 13, 2020 |
| Title |
Fraction 6#1 |
| Sample type |
SRA |
| |
|
| Source name |
Fraction 6 B cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: spleen
genotype: Bcl6-YFP(KI/+) B1-8hi(KI/+)
cell type: Fraction 6 B cells (pre-memory B cells)
|
| Growth protocol |
Bcl6-YFP B1-8hi naïve B cells were transferred into wild-type CD45.1+ mice. Mice were immunized i.p. (day 0) with NP-CGG/alum and NP-specific IgG1+ donor GC LZ (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4lo), Fr.2 (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4loBcl6-YFPhiCD69hi), Fr.3 (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4loBcl6-YFPhiCD69lo), Fr.5 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPhiEfnb1hi), Fr.6 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPloEfnb1hi), and Fr.7 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPloEfnb1lo) B cells were sorted from pooled splenocytes of 9 recipient mice on day 10.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Invitrogen).
DNA library was constructed using an NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) from total RNA purified from 1~2 × 10^4 sorted cells.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Read alignment: TopHat2 v2.0.8 with default settings
expression level: Cufflinks v2.1.1 with default settings
Genome_build: mm9
Supplementary_files_format_and_content: pro-mem RNA-seq.txt
|
| |
|
| Submission date |
Mar 17, 2020 |
| Last update date |
Sep 13, 2020 |
| Contact name |
Takeshi Inoue |
| Organization name |
Osaka University
|
| Street address |
3-1 Yamadaoka
|
| City |
Suita-shi |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (2) |
| GSE147095 |
mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center [2] |
| GSE147096 |
mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center |
|
| Relations |
| BioSample |
SAMN14390991 |
| SRA |
SRX7924363 |
