|
Status |
Public on Sep 13, 2020 |
Title |
LZ#1 |
Sample type |
SRA |
|
|
Source name |
GC LZ B cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: spleen genotype: Bcl6-YFP(KI/+) B1-8hi(KI/+) cell type: GC LZ B cells
|
Growth protocol |
Bcl6-YFP B1-8hi naïve B cells were transferred into wild-type CD45.1+ mice. Mice were immunized i.p. (day 0) with NP-CGG/alum and NP-specific IgG1+ donor GC LZ (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4lo), Fr.2 (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4loBcl6-YFPhiCD69hi), Fr.3 (CD45.2+NP+CD138−IgG1+CD38−CD86hiCXCR4loBcl6-YFPhiCD69lo), Fr.5 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPhiEfnb1hi), Fr.6 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPloEfnb1hi), and Fr.7 (CD45.2+NP+CD138−IgG1+CD38+GL7−Bcl6-YFPloEfnb1lo) B cells were sorted from pooled splenocytes of 9 recipient mice on day 10.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Invitrogen). DNA library was constructed using an NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) from total RNA purified from 1~2 × 10^4 sorted cells.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Data processing |
Read alignment: TopHat2 v2.0.8 with default settings expression level: Cufflinks v2.1.1 with default settings Genome_build: mm9 Supplementary_files_format_and_content: pro-mem RNA-seq.txt
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|
|
Submission date |
Mar 17, 2020 |
Last update date |
Sep 13, 2020 |
Contact name |
Takeshi Inoue |
Organization name |
Osaka University
|
Street address |
3-1 Yamadaoka
|
City |
Suita-shi |
State/province |
Osaka |
ZIP/Postal code |
5650871 |
Country |
Japan |
|
|
Platform ID |
GPL18480 |
Series (2) |
GSE147095 |
mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center [2] |
GSE147096 |
mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center |
|
Relations |
BioSample |
SAMN14391102 |
SRA |
SRX7924351 |